Pepsin hydrolysis of bovin β-lactoglobulin under microwave reduce its allergenicity as studied ex vivo in murine model of allergy
نویسندگان
چکیده
منابع مشابه
In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization
OBJECTIVE To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick t...
متن کاملextraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولcomparison of immunomodulating properties of beta-lactoglobulin and its hydrolysates.
cow’s milk allergy is one of the most common food allergies in childhood. beta- lactoglobulin (β- lg) is a dominant allergen in cow’s milk. hydrolysis is known as an effective method to reduce the allergenicity of proteins. thus, the objective of this study was to compare the allergenicity of β-lg and its hydrolysates using an animal model. twenty four balb/c mice were divided into three ...
متن کاملComparison of immunomodulating properties of Beta-lactoglobulin and its hydrolysates.
Cow's milk allergy is one of the most common food allergies in childhood. Beta-lactoglobulin (β- lg) is a dominant allergen in cow's milk. Hydrolysis is known as an effective method to reduce the allergenicity of proteins. Thus, the objective of this study was to compare the allergenicity of β-lg and its hydrolysates using an animal model. Twenty four BALB/c mice were divided into three groups ...
متن کاملRole of specific IgE to β-lactoglobulin in the gastrointestinal phenotype of cow’s milk allergy
RATIONALE The prevalence of many phenotypes of food allergy is increasing. Specific gastrointestinal (GI) phenotype of food allergy (GI allergy) is also increasing but it is difficult to know the prevalence because of many entities. METHODS AND RESULTS: A 1 year retrospective study of pediatric patients complaining exclusively gastrointestinal symptoms after cow's milk consumption and at least ...
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ژورنال
عنوان ژورنال: Proceedings of the Nutrition Society
سال: 2013
ISSN: 0029-6651,1475-2719
DOI: 10.1017/s0029665113000414